Fig. 5. Inhibition of Rho-kinase or nonmuscle myosin II results in decreased marginal actin bundle and stress fiber numbers and staining intensity. MDCK cell monolayers (1:10 ratio of GFP-actin expressing cells and nontransfected cells) were fixed, permeabilized and stained for F-actin with TRITC-phalloidin 6 hours after wounding following treatment with (A) 0.2% DMSO carrier solvent, (B) 50 µM H1152, a Rho-kinase inhibitor, or (C) 100 µM (±)-blebbistatin, a nonmuscle and skeletal muscle myosin II inhibitor. The initial width of the rectangular wounds was 700 µm. Similar results were obtained with Y-27632, another Rho-kinase inhibitor, and ML-7, a myosin light chain kinase inhibitor. Marginal actin bundles are indicated by asterisks, whereas membrane protrusions are indicated by arrows. Submarginal protrusions are also discernable in these images. Images are representative of at least three independent experiments in each case. Bar, 25 µm.