Fig. 7. Chk1 contains a functional NES. (A-D) Chk1 immunofluorescence was performed on strains harboring the indicated plasmid-borne Chk1 alleles after treatment with or without 100 ng/ml LMB for 1-2 hours. Quantification was then performed (an average of four independent experiments is shown for the wild-type, three independent experiments for each of the mutants). Bar, 10 µm.