Fig. 3. Cx45 gene expression in regenerating tibialis anterior muscles (TA). (A) Eosin stained TA cross sections of control Cx45^+/– mice showed X-gal reactivity in microvessels (empty arrowhead). At day 3, numerous cells showed cytoplasmic X-gal positive dots (open arrowheads). At 7 days PI X-gal positive dots were found within (arrows) and between (open arrowheads) fibers. (B-D) Co-localization of X-gal staining with specific cell markers in regenerating TAs from Cx45^+/– mice. Arrows indicate sites of co-localization; both arrow and arrowheads indicate positive X-gal staining. These panels show co-localization of von Willebrand factor (VWF) with X-gal staining under control conditions and at D3 PI (B), with CD14 at D3 PI (C) and with myogenin (Myo) at D5 PI (D). Scale bars: In A, 100 µm (left column) and 50 µm (right column); in B, 45 µm (upper panels), 30 µm (middle panels) and 50 µm (bottom panels); (C,D) 20 µm.