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Fig. 2. Localization of MT1-MMP in bone. (A) Bright-field image of a bone section from a 20-day-old wild-type mouse hybridized to MT1-MMP-antisense RNA probe. Staining is intense in the periosteum (p), with multiple cells expressing MT1-MMP-encoding mRNA. Isolated osteocytes in the bone cortex (c) likewise display signal indicating expression of MT1-MMP. e, endosteum. (B) Serial section from the same animal hybridized with MT1-MMP-sense RNA probe. (C) Immunolocalization of MT1-MMP in cortical bone from a 30-day-old wild-type mouse demonstrating osteocytes and their cell processes expressing MT1-MMP. (D) Bone section from 40-day-old wild-type mouse demonstrating even more intense stain than that seen at day 30. (E,F) Negative controls. Bone sections from 30-day-old MT1-MMP-deficient mouse stained for MT1-MMP (E) and 40-day-old MT1-MMP-deficient mouse stained for MT1-MMP (F). Bars, 40 µm (A,B), 10 µm (C-F).