Fig. 2. Altered morphology in mdx^3cv mouse spermatozoa. (A) Flagellar morphology patterns from wild-type and mdx^3cv mice were analyzed and quantified by phase-contrast microscopy. Different sperm samples of both strains presented the classic normal morphology (A1), as well as two different abnormalities: curved (A2) and twisted flagella (A3). (B) Quantification of flagellar morphology patterns, normal shape (B1), curved (B2) and twisted flagella (B3). (C) Quantification of flagellar morphology patterns of Brij-36T treated spermatozoa: normal flagella (C1), curved flagella (C2) and twisted flagella (C3). Black bars for wild-type mice and white bars for mdx^3cv mice. The data in these graphs represent the mean±s.e. from five independent experiments. Bar, 6 µm.