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Fig. 1. Graphical presentation of cDNA constructs for membrane-bound HB-EGF (proHB-EGF), soluble HB-EGF (sHB-EGF)and the deletion mutant constructs. The sequence of the juxtamembrane extracellular domain and the amino acids deleted in the mutant constructs are shown. A C-terminal FLAG epitope (M2) was added to each construct.