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Fig. 7. Laminin 5 deposits and integrin {alpha}3ß1 mediate adhesion to BSA. Activated keratinocytes were plated onto a BSA blocked surface for 4 hours and the percentage adhered cells relative to nontreated control (nt=1.0) was determined. mAb C2-9 against laminin 5 (lm5) blocked adhesion, and JEB-gravis cells, which lack laminin 5, failed to adhere. Nocodazole and taxol at 1 µM and rotterlin at 6 µM completely blocked adhesion. Inhibitory antibodies against integrin ß1 (P5D2) and integrin {alpha}3 (P1B5) inhibited adhesion while antibodies against integrin {alpha}6 (goH3) did not. JEB-PA cells lacking integrin ß4 were able to adhere to BSA. Cytochalasin D at 5 µM did not block adhesion. Data shown is the average and standard deviation of three independent experiments. *Significantly different from nt value, P<0.01; Student's t-test.