Fig. 5. Laminin 5 provided a better substratum for processive migration than did collagen. Cells were plated onto exogenous laminin 5 or collagen for 1-2 hours before migration was recorded at 37°C by time-lapse imaging every 2 minutes for a total of 2 hours. (A) Plots of cells on exogenous laminin 5 (a) or collagen (b) showing 2 minute interval positions for all cells in one 10x field oriented so that cell origins are at x(0), y(0). Distance migrated in microns is indicated on the plots. (B) Processive index for each cell in A is shown as a function of the total distance traveled for cells on laminin 5 (a) or collagen (b). Shaded regions indicate processively migrating cells with a total migration >40 µm and PI>0.7.