Fig. 4. Activated keratinocytes on laminin 5 assembled small, punctate focal complexes during processive migration. Cells were plated onto exogenous laminin 5 (A-F) or collagen (G-L) for 2 hours, then fixed and stained for immunofluorescence. Deconvoluted optical sections of the basal cell surface are shown. Phospho-FAK-Y397 (red) (A,D,G,J), ß1 integrin mAb 9EG7 (B) and 3 integrin mAb P1F2 (E,K) and 2 integrin mAb P1H5 (H) (green). Scale bar: 10 µm.