Fig. 2. Polarization is affected by exogenous ligand and requires growth factors, PI 3-kinase and protein translation. Keratinocytes were plated for 2 hours on laminin 5 (black bars) or collagen surfaces (white bars) with either no treatment (nt), absence of growth factors (–GF), presence of 5 µM LY294002 (LY29) or presence of 10 µg/ml cycloheximide (CHX). Values represent the average and standard deviation of at least three independent experiments.