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Fig. 3. Ezrin is decreased in the absence of Foxj1. (A) Western blot of E15.5 lung tissue with primary antibody to ezrin. Ezrin is an 80/81 kDa protein. Comparison was made to GAPDH (36 kDa) to correct for protein loading. (B) RT-PCR for ezrin expression in E15.5 lung tissue. A 170 bp ezrin product is detected in both foxj1+/+ and foxj1-/- lung tissue. (C,D) Immunofluorescence of foxj1+/+ (C) and foxj1-/- (D) in mouse trachea with primary antibody to ezrin or acetylated {alpha}-tubulin and detection of fluorescence. Scale bar: 10 µm.