Fig. 3. Kisspeptin-10 raises intracellular Ca²⁺ in isolated first trimester human trophoblasts. FT trophoblasts were stimulated with different Kps and intracellular [Ca²⁺] was measured (A). Only Kp-10 resulted in an increase in intracellular [Ca²⁺] (n=9), whereas Kp-13, -14 and -54 were ineffective (n=6). (B) Concentration-response curve for Kp-10 on intracellular free Ca²⁺ concentration in isolated FT trophoblasts. The intracellular [Ca²⁺] is expressed as ratio (F₃₄₀/F₃₈₀) (mean±s.e.m.; n=6-9). The EC₅₀ was found to be 21 (16-29) nM (95% confidential interval).