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Fig. 3. {alpha}-Synuclein expression does not perturb axonal transport of mitochondria in rat cortical neurons. Rat primary neuronal cortical cultures (5 DIV) were transfected with a plasmid expressing the targeting sequence from subunit VIII of cytochrome c oxidase tagged with DsRed2 (MITO) or co-transfected with the mitochondrial marker plus EGFP-tagged wild-type (WT) or A30P mutant {alpha}-synuclein (A30P), and fixed 24 hours after transfection. Protein expression was detected by direct fluorescence of DsRed2-cytochrome c oxidase (MITO), EGFP-tagged wild-type (WT) or A30P mutant (A30P) {alpha}-synuclein. Scale bar, 50 µm.