JCS -- Liang et al. 117 (5): 723 Figure IG8

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Fig. 8. Subcellular fractionation of control and agonist-treated cells by sucrose density gradient centrifugation. Arr2-BHK-hß₁ (A,C) and -hß₂ (B,D) cells were induced with zinc sulfate for 24 hours. Lysates prepared from control cells (open symbols) and cell treated with ISO for 15 minutes (closed symbols) were fractionated by sucrose density gradient centrifugation and the fractions assayed for binding with ¹²⁵ICYP ( ${circ}$ , ${square}$ , ${bullet}$ , ${blacksquare}$ ) and [³H]CGP-12177 ( ${triangleup}$ , ${triangledown}$ , ${blacktriangleup}$ , ${blacktriangledown}$ ) along with the sucrose concentration ( ${diamond}$ , ${diamondsuit}$ ) as described in Materials and Methods. Distributions of total receptors between plasma membrane and endosomal fractions were for ß₁AR: control, 82.7±0.6 and 17.3±0.6%; ISO-treated, 63.1±3.6 and 36.9±3.6% (n=4); and for ß₂AR: control, 87.8±2.4 and 12.2±2.4%; ISO-treated, 49.0±1.1 and 51±1.1% (n=3).