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Fig. 6. Electron microscopy (A,B) and light microscopy (C,D) of human control liver. (A) Ultrathin Unicryl sections of human liver were immunostained with the affinity purified antibodies against MK. The peroxisomes (P) remain unlabelled. (B) Ultrathin Unicryl sections immunostained with antibodies against against AGT reveals a clear localisation in the peroxisomal matrix. Bar, 500 nm; M, mitochondria. (C) Cryostat sections immunostained with affinity purified antibodies against MK (silver enhancement of colloidal gold) reveals a diffuse reaction in the cytosol of the hepatocytes. For comparison, immunostaining with antibodies against AGT reveals a distinct granular pattern, reflecting a peroxisomal localisation of AGT (D).