Fig. 5. Localization of Ace2p in wild-type and swm1 mutants. (A) Diploid wild-type (YPA24) cells carrying the Ace2-YFP or Ace2-G128EYFP fusion proteins were grown at 38°C for 4 hours and stained with DAPI. (Left) Differential interference contrast microscopy (DIC); (middle) YFP fluorescence; (right) DAPI fluorescence. (B) Diploid wild-type (YPA24) or swm1 (YPA207) cells were transformed with plasmid pACE2-YFP and the localization of the protein was determined by fluorescence microscopy during growth at 28°C or after 2 hours or 8 hours of incubation at 38°C. (Left) Differential interference contrast microscopy; (right) YFP fluorescence. (C) Localization of Ace2-G128Ep in wild-type and swm1 mutants. Diploid wild-type (YPA24) or swm1 (YPA207) cells were transformed with plasmid pACE2-G128E-YFP and the localization of the protein was determined by fluorescence microscopy during growth at 28°C or after 4 hours of incubation at 38°C. (Left) Differential interference contrast microscopy; (right) YFP fluorescence.