Fig. 3. Polarized subcellular distribution of PCP genes in wing and eye cells. (A) Pupal wing cells show a transient accumulation of PCP proteins on either the proximal or distal cell membranes. Fz and Dsh accumulate on the distal membranes, Stbm and Pk accumulate on proximal membranes, and Fmi and Diego appear to be enriched on both proximal and distal membranes. All are depleted from anterior and posterior membranes. (B) PCP proteins accumulate specifically on subsets of ommatidial precursor cells, most importantly at the R3/R4 interface, shown here. Fz and Dsh accumulate on the R3 cell at the R3/R4 interface, whereas Stbm is enriched in the R4 cell at the R3/R4 interface. Other photoreceptor cells (not shown) show polarized distributions of some components, but importantly cell in-between ommatidial clusters show no polarized accumulation of PCP proteins.