Fig. 7. ODF2 does not bind directly to tubulin. Purified ODF2-GST fusion proteins were incubated with purified tubulin under conditions that promote microtubule formation. GST fusion proteins were isolated by affinity chromatography, separated using denaturing SDS-PAGE, transferred to Hybond-C, and probed with anti-ODF2 antibodies (A), and anti--tubulin antibodies (B). Whereas ODF2 fusion proteins were successfully isolated, -tubulin was not coprecipitated.