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Fig. 6. Copurification of ODF2 with tubulin from Xenopus oocyte extracts. Purified ODF2NC-GST or ODF2NC2-GST proteins were incubated in Xenopus oocyte extracts supplemented with tubulin and under conditions that promote tubulin polymerization. GST fusion proteins and associated proteins were isolated by affinity chromatography, separated using denaturing SDS-PAGE, transferred to Hybond-C and probed with anti-ODF2 antibodies (A), and anti-{alpha}-tubulin antibodies (B).