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Fig. 6. Cru1 is required for full virulence. (A) Tumors produced by wild-type strains after 2 weeks of infection. (B) Tumors produced by cru1{Delta} cells after 2 weeks of infection. Further incubation does not result in an increase in tumor size. (C) Section of a 3-week tumor produced by wild-type strains. A massive production of mature teliospores can be observed. The inset shows a higher magnification of teliospores. Bar, 10 µm. (D) Section of a 3-week tumor produced by cru1{Delta} cells. Very few mature teliospores can be observed and frequently they have an anomalous morphology (inset showing a higher magnification. Bar, 10 µm).