Fig. 6. Treatment with 300 µM ryanodine abolishes caffeine induced Ca²⁺ release in germ cells. Cells were preloaded with 10 mM CaCl₂, washed and either incubated (A) with or (B) without 300 µM ryanodine for 1 hour with before measuring caffeine-dependent Ca²⁺-release. Ionomycin (IMC) was added to elicit further release of intracellular Ca²⁺. The traces are typical of three independent measurements.