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Fig. 3. Analysis of RyR3 expression by western blotting and RTPCR. (A) Immunoblot of 100 µg microsomal proteins extracted from diaphragm (D), 60-day-old testis (T60), mixed germ cells (gc) probed with antibody against RyR3. {alpha}-RyR3 recognized a single specific band in diaphragm and a lower molecular weight band in testis and germ cells, which was proven to be nonspecific. (B) RyR3 detection by RT-PCR using isoform-specific primers that gives a single 505 bp product. Lane marked `-' is the negative control.