Fig. 2. RyR2 expression by western blotting and RT-PCR in the developing testis and isolated germ cells. (A) Immunoblot of microsomal proteins prepared from adult heart (10 µg), and total testis (100 µg) at different ages of postnatal development between 10 and 60 days. (B) RT-PCR analysis of RyR2. Using isoform-specific primers, a single 635 bp product was identified in 7-day-old testis (T7) and in an enriched (approximately 60%) population of spermatogonia (spg^*), but not in highly purified spermatogonia (spg) and in Sertoli cells (SC). Lane marked `-' is the negative control.