Fig. 7. Effect of Src on scratch-wound closure in A6(1) cells. (A) Scratch wounds were made in confluent cultures of A6(1) cells. (B) After 24 hours in culture, the wound was largely occupied by migrating cells. (C) Scratch wounds were made in confluent cultures of A6(1) cells in the presence of the Src-family-kinase inhibitor PP1. (D) After 24 hours in the presence of PP1, very few cells had migrated into the wound area. The dark spots in the lower left quadrant of each panel are ink spots used to identify the region of interest. (E) Quantitative image analysis of the proportion of the original wound area occupied by migrating cells at the end of 24 hours in the absence or presence of PP1. In three experiments, the occupied area decreased from 54±6% to 7.5±1.6%. The difference between the groups is significant (P<0.001).