Fig. 5. Neurite outgrowth is reduced on neuraminidase treated embryonic astrocytes. Neurons were seeded onto confluent E16 astrocyte monolayers either non-treated (A) or digested with neuraminidase and allowed to recover for 1 hour (B). Arrows in panel B point to cell clusters similar to those formed by neurons plated on P0 monolayers (see Fig. 2). Panels C and D show quantitative analyses of data obtained in panels A (black bars) and B (white bars). Quantification was performed for 100 neurons randomly chosen. ^*P<0.001. Bar, 50 µm.