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Fig. 3. Astrocytes derived from E16 or P0 present different surface potentials. After mechanical dissociation of monolayers, astrocytes derived from E16 ({square}) or P0 ({blacktriangleup}) had their zeta ({xi}) measured by cell electrophoresis. Values of Pzeta at different pHs are plotted in the main panel. The inset shows values of {xi} measured immediately after treatment with neuraminidase and 30 or 60 minutes after removal of the enzyme. *P<0.01; **P<0.001.