Fig. 2. Reporter genes activated by mitochondrial perturbation. (A) Fluorescence photomicrographs of untreated (UT), ethidium bromide (EtBr; µg/ml) or tunicamycin-treated (Tun; 1 µg/ml) or heat-shocked (HS) animals with reporter transgenes for mitochondrial chaperones (hsp-6::gfp, hsp-60::gfp), an endoplasmic reticulum chaperone (hsp-4::gfp), a cytoplasmic chaperone (hsp-70::gfp), and the mitochondrial tri-carboxylic acid cycle enzymes citrate synthetase (cts-1::gfp) and aconitase (aco-2::gfp). (B) Immunoblot of soluble proteins extracted from the hsp-6::gfp animals described in A. The blot was reacted with anti-GFP serum (upper panel) or antiserum to the broadly expressed UNC-32 protein (lower panel). (C) Northern blot of untreated and ethidium bromide-treated hsp-6::gfp animals. The blot was hybridized sequentially with a hsp-6-coding region probe that detects the endogenous gene and a GFP probe that detects the transgene.