Fig. 1. Attenuated function of the mitochondrial genome induces mtHSP70 (hsp-6) mRNA. (A) Autoradiogram of a Southern blot of EcoRI-digested total DNA from animals raised in the indicated concentration of ethidium bromide (EtBr; µg/ml), or tunicamycin (Tun; 1 µg/ml). The blot was hybridized sequentially to a labeled DNA fragment of the mitochondrially encoded cox-1 gene and the nuclear encoded K08H10.2a gene. (B) Autoradiogram of the northern blot of total RNA from animals cultured on agar plates containing the indicated concentration of ethidium bromide or tunicamycin. (Upper panel) The blot was hybridized to a labeled cox-1 DNA fragment. (Lower panel) Ethidium bromide staining of the ribosomal bands of the RNA. (C) Autoradiogram of a northern blot of total RNA from animals treated with ethidium bromide, tunicamycin or heat-shock (HS). The blot was hybridized sequentially to radiolabled fragment from the mtHSP70 gene (hsp-6) and the C. elegans BiP homologue, hsp-4. Bar charts on the right of each panel show the relative intensity of the hybridization signal.