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Fig. 8. Requirement for cellular kinase activity in Daxx and SSRP1 accumulation at heterochromatin. Relative to control (a), Daxx after roscovitine treatment of cells (d) shows no heterochromatic accumulation but it remains localized with PML. (b,e) ATRX and PML remain colocalized after roscovitine treatment and ATRX still accumulates at heterochromatin. The colocalization of Daxx and SSRP1 in heterochromatin in control cells (c, upper left) is absent from roscovitine-treated cells (e). (g) MPEF cell extracts were prepared without treatment (control lane), and 1 hour after roscovitine treatment; untreated (lane control 1) or protein-phosphatase-treated (lane phosphatase). Although Daxx, PML and HP1{alpha} did not change mobility after roscovitine treatment, the new ATRX band (*) migrates similarly to ATRX after phosphatase treatment.