Fig. 7. Cell-cycle-dependent intranuclear distribution of SSRP1. (a) Cells double-labeled for SSRP1 and PML show that SSRP1 does not accumulate at ND10 but is present at irregular domains in some cells (upper left and right cells). (b) Double-labeling for SSRP1 and HP1 reveals SSRP1 at heterochromatin in some cells (two cells at lower right). (c) Double-labeling for Daxx and SSRP1 demonstrates co-localization of the proteins in heterochromatic domains (upper middle cell). (d) Cells pulsed with BrdU and labeled for SSRP1 and BrdU show homogeneous nuclear distribution of SSRP1 in most cells but localization with the late BrdU incorporation pattern (S-late). (e) Cells labeled for SSRP1 and phosphorylated histone H3 shows accumulation of SSRP1 at heterochromatin at the beginning of histone H3 phosphorylation corresponding to late S-phase and early G₂ phase (S/G2) but not late G₂ phase (G2). (f) The phosphorylated histone H3 signal shown separately.