Fig. 2. Cell-cycle-dependent distribution of Daxx. (a-c) MPEF labeled for phospho-histone H3 and Daxx show that no Daxx accumulates at heterochromatin during G₁, G₂ or M phase. (d-g) MPEF pulsed with BrdU for 30 minutes and triple-labeled for BrdU, ND10 and Daxx show Daxx located at heterochromatin during late S-phase but not during middle S-phase. (h-j) MPEF pulsed with BrdU for 30 minutes, chased for 3 hours and double-labeled for BrdU and Daxx show Daxx in ND10-like domains but not in heterochromatin of the cell that was at late S-phase during the pulse, and on heterochromatin in cell that was at early S-phase during the pulse. (k-m) PML^–/– cells pulsed with BrdU for 30 minutes and labeled for BrdU and Daxx show Daxx concentrated at heterochromatin only during late S-phase despite the absence of ND10.