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Fig. 1. Daxx is a chromatin-associated protein. (a) MPEF double labeled for PML (red) and Daxx (green). Both proteins localize at ND10, and Daxx also accumulates in a different shaped domain (upper right nucleus). (b) MPEF double labeled for HP1{alpha} and Daxx. Daxx localizes with HP1{alpha} at heterochromatin (middle right). (c) In PML–/– cells, Daxx accumulates in heterochromatin-like domains that localize with HP1{alpha} (d). (e) Western blot of intact MPEF cells (lane 1), cytoplasm (lane 2), nuclear insoluble fractions (n.p.) digested by micrococcal nuclease (MNase) (lane 3) and control (lane 4). Treatment with MNase resulted in the complete solubilization of Daxx and HP1{alpha} (lane 3). Loads in lanes 1 and 2 correspond to 10% of cells used in lanes 3 and 4. The asterisk indicates SUMOylated PML.