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Fig. 5. Effect of previous nicking of DNA or inhibition of transcription on psoralen signals on heat-shock puffs. Salivary glands dissected from heat-shocked larvae were irradiated with X-ray (A-C) or treated with {alpha}-amanitin (D-F) and then photocrosslinked with biotinylated psoralen. (A,D) Biotinylated psoralen. (B,E) DAPI. (C,F) Merged. Arrows indicate heat-shock puffs at 87A and 87B. Bars, 10 µm.