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Fig. 4. Distribution of psoralen signals on polytene chromosomes from heat-shocked larvae. (A) Biotinylated psoralen. (B) DAPI. (C) Merged. Major heat-shock puffs at 87A, 87B and 93D are indicated by arrows. Arrowheads represent interbands with biotinylated psoralen signals that became less prominent after heat shock. Bar, 10 µm.