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Fig. 3. Bright psoralen signals disappear upon previous nicking of DNA or inhibition of transcription. Salivary glands were irradiated with X-rays for relaxation of DNA by nicking (A-C) or treated with {alpha}-amanitin for inhibition of transcription (D-F) before photocrosslinking with biotinylated psoralen. (A,D) Biotinylated psoralen. (B,E) DAPI. (C,F) Merged. Representative interbands and puffs are indicated by arrows. Bars, 10 µm.