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Fig. 6. GEF activity of PLC-{gamma}1 for dynamin-1 upregulates dynamin-dependent ERK activation. PC12 cells transfected with various PLC-{gamma}1 constructs (A), PLC-{gamma}1 siRNA duplexes (#1 and #2) (B) or the SH3 domains of PLC-{gamma}1 and amphiphysin (C) were treated with EGF for indicated times. Activation of ERK was measured by immunoblotting with phospho-ERK1/2 antibody. The total cell lysates were analyzed by immunoblotting with ERK1/2 antibody.