Fig. 5. PLC-1's GEF activity potentiates dynamin-1-dependent EGFR endocytosis. PC12 cells transfected with various PLC-1 constructs (A) or PLC-1 siRNA duplexes (B) were incubated with Rhodamine-conjugated EGF at the indicated times and fixed cells were examined by confocal microscopy. Arrowheads indicate the internalized EGF-EGFR vesicles.