Fig. 8. Effect of PMA on tyrosine phosphorylation and motility of several non-adherent (32D, CCRF CEM, Daudi) and adherent (Swiss 3T3, COS-1) cell lines maintained in complete medium. (A) After a 15-minute treatment with 100 nM PMA, paxillin was immunoprecipitated and analyzed by western blotting with a mixture of anti-PY20 and 4G10 antibody (IB pY) or with anti-paxillin antibody (IB Paxillin). (B) Spontaneous cell motility was analyzed as described in the Materials and Methods. The mean and s.e.m. from six experiments are presented. PMA inhibited the motility of 32D cell line (t=9.2, P=0.0003) and CCRF CEM cell line (t=8.4, P=0.0004), increased the motility of Swiss 3T3 (t=–4.16, P=0.0088) but did not significantly affect the motility of Daudi (t=1.1, P=0.33) or Cos1 cells (t=–0.49, P=0.64).