Fig. 7. Tyrosine phosphorylation of paxillin residues 31 and 118 does not affect its intracellular distribution in Baf3 cells. Parental Baf3 cells (A) and Baf3 cells expressing EGFP (vector), EGFP-conjugated wild-type paxillin or YF tyrosine phosphorylation mutant 31/118 (B) were maintained on 10 ng/ml IL-3. Endogenous paxillin was visualized by the consecutive staining with monoclonal anti-paxillin and fluorescein-conjugated goat anti-mouse secondary antibody (A). DIC images were collected using transmitted light; intracellular distribution of endogenous paxillin (A) and EGFP-paxillin (B) was analyzed by fluorescent confocal microscopy. Bars, 10 µm. Thus, we show similar distribution of endogenous paxillin, EGFP-conjugated wild-type paxillin and its mutants.