Fig. 6. Regulation of ppMLC^T18/S19 by cell adhesion to FN. (A) Human fibroblasts were detached, resuspended in 10% FBS and immediately plated onto dishes coated with 20 µg ml^–1 FN for the indicated times for the analysis of ppMLC^T18/S19 and pMBS^T853. (B) Human fibroblasts were suspended in 1% FBS for 60 minutes and plated onto dishes coated with 20 µg ml^–1 FN or PLL for indicated times. (bottom) NIH3T3 cells transiently expressing RhoA^V14 or Rho-kinase CAT were suspended in serum-free medium for 30 minutes and plated onto dishes coated with 20 µg ml^–1 FN or 100 µg ml^–1 PLL for 30 minutes for the analysis of ppMLC^T18/S19.