Fig. 4. pMBS^T696 and pCPI-17^T38 levels are not significantly affected by detachment, serum starvation or Y-27632 treatment. (A) Cells were detached and incubated for 3 minutes in the presence of serum (Det), attached (Att) in the presence of serum, serum starved for 30 minutes or treated with 10 µM Y-27632 for 30 minutes in the presence of serum. Data from four independent experiments were summarized and presented as mean±s.e.m. relative to the serum-fed attached cells. (top right) pMBS^T696 decreased in COS-7 cells treated with 10 µM Y-27632 for 30 minutes in the presence of serum. (B) Cells were treated the same way as above for the detection of pCPI-17^T38. (right) Fibroblasts were treated with 0.5 µM PMA or 1:2000 DMSO for 30 minutes.