Fig. 5. Cell aggregation assays to test the adhesive specificities of Bb1C and Bb2C. (A-C) S2 cells transiently transfected with plasmids for Bb1C-GFP (A,B) and no insert (C) were tested for aggregation in the presence of 1 mM Ca²⁺ (A,C) or 1 mM EDTA (B). The cells expressing Bb2C-GFP were aggregated in a Ca²⁺-independent manner. (D) S2 cells separately transfected with plasmids for Bb1C and Bb2C-GFP were mixed and tested for aggregation. The resulting aggregates were fixed and stained for Bb1C (red). The cells expressing Bb1C (red) and those expressing Bb2C-GFP (green) aggregated separately.