Fig. 2. FHOS associates with the actin cytoskeletion. (a) FHOS co-localizes with actin in the cytoplasm and in protrusions. Transfected 293T cells expressing full length FHOS fused to EGFP, FHOS (green, panels 1-3, 6) were co-stained with either TRITC-phalloidin (panel 4; red) to detect actin or with anti -tubulin/goat F(ab')₂ anti-mouse Ig R-PE (panel 7; red). Merged images (panel 3 with 4 and panel 6 with 7) are shown in panels 5 and 8, respectively. Hoechst 33342 (blue) was used to detect nuclei in panels 5 and 8. (b) FHOS accumulates with actin at the border between cells and without actin in a perinuclear patch. Transfected 293T cells expressing EGFP-FHOS (green; panels 1,4-5) were co-stained with TRITC-phalloidin (panels 2 and 6; red) and merged images (panel 1 with 2 and panel 5 with 6) are shown in panels 3 and 7, respectively. Nuclei are stained blue as in a. All cells were fixed, stained and imaged using an Eclipse E600 fluorescence microscope and processed using Spot Advance technology.