Fig. 7. Distribution/localization of Cx37 is not affected by BFA treatment. Cultured COCs were treated with 10 µg/ml of BFA for 5 hours to block connexin transport and gap-junction plaque regeneration. Immunostaining for anti-giantin (A, red) revealed that the Golgi apparatus was effectively disrupted upon BFA treatment (C, red). However, Cx43 gap-junction plaques between adjacent granulosa cells (A,C; anti-Cx43, green) and Cx37 at oocyte-granulosa cell interfaces (B,D; anti-Cx37, red) were not markedly altered by BFA treatment. To enhance cellular architecture, COCs were double-labelled with a non-specific antibody that provided background immunofluorescence (B,D, green). Bars=10 µm (A,C); 25 µm (B,D).