Fig. 5. Decoration of DAPI-positive heterochromatin blocks with anti-Me(3)K20H4 antibody in C127 cells is enhanced upon serum removal. C127 cells were cultured in normal culture medium (A,B) or in culture medium containing 0.4% (C,D) and 0.1% (E,F) foetal calf serum. (A,C,E,G) Staining with anti-Me(3)K20H4; (B,D,F,H) DAPI profiles of the same fields. One heavily decorated nucleus, typically found in cells cultured in low serum concentration, is shown in G and H. Scale bars: 10 µm (A-F); 5 µm (G,H)