Fig. 4. Me(3)K20H4 replicates late in S-phase of the cell cycle. C127 cells were arrested at the G1/S border with mimosine and then released into S-phase. Cells were pulse labelled with BrdU either early (2 hours after release, S-E) or late (8 hours after release; S-L) in S-phase, and were then stained for DAPI (blue), BrdU (red) and Me(3)K20H4 (green). In the far right column, the BrdU and Me(3)K20H4 staining patterns were simultaneously visualised through a dual FITC/Texas Red filter (Dual Filter). This showed that the BrdU and Me(3)K20H4 staining patterns colocalise late in S-phase (S-L). Scale bar: 5 µm.