Fig. 8. The effect of PSA removal on PDGF-induced lamellipodial formation. Control and Endo N-treated (12 hours) cells were serum starved and incubated with medium (A,B), 0.5 ng/ml PDGF (C-F) or 5 ng/ml PDGF (G,H) for 10 minutes. Cells were fixed, stained with Texas Red-phalloidin and Oregon Green DNase I to detect F-actin fibers and G-actin, respectively. Cells were examined by fluorescence confocal microscopy. Bar, 20 µm in A-D,G and H; 8 µm in E and F.