Fig. 4. Phylogenetic analysis of ENTH domains. Amino acid sequences of ENTH-bearing proteins from human, fly, worm and yeast were retrieved from the GenBank database (Hs-Epsin1, NP_037465; Hs-Epsin2a, AAC78608; Hs-Epsin3, AAG45223; Hs-Enthoprotin, DAA00062; Ce-Liquid facets, NP_510458; Ce-Epsin, NP_509973; Dm-Liquid Facets, AAF05113; Dm-Enthoprotin, AY060606; Sc-Ent1p, NP_010120; Sc-Ent2p, NP_013307; Sc-Ent3p, NP_012659; Sc-Ent4p, NP_013062; Sp-NP_587759; Sp-NP_588237). Sequences of the ENTH domains were aligned using ClustalW. The alignment was then used for phylogenetic comparisons using the PHYLIP package [J. Felsenstein, 1993, PHYLIP (Phylogeny Inference Package) 3.6a3; University of Washington, Seattle]. Analysis was performed with a bootstrap procedure that computes the probability of occurrence of the branches for 100 possible trees. Branching order was determined using the Jones-Taylor-Thornton model included in the PHYLIP package. The tree was rooted by the most divergent sequence, Sc-Ent4p, as the outgroup. Bootstrap values are shown at each node. The tree visualization was performed using TreeView 1.6.6 (Page, 1996). Hs, Homo sapiens; Dm, Drosophila melanogaster; Ce, Caenorhabditis elegans; Sc, Saccharomyces cerevisiae; Sp, Schizosaccharomyces pombe.