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Fig. 4. Subcellular localisation of endogenous PKC {alpha} and {epsilon} in unstimulated and TPA-stimulated GH3B6 cells. Cells were incubated in the presence of 100 nM TPA for 1 hour and immunostained as described in Materials and methods. PKC {alpha} and {epsilon} are selectively accumulated at the cell-cell contacts in the presence of TPA.