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Fig. 5. Localization of X-gal staining in sections of normal blastocysts or trophoblast vesicles formed in vitro from two-cell ROSA26 embryos. (a) Section of a control blastocyst transferred to the oviduct for sectioning, and (b) section of a trophoblast (Tr) vesicle transferred to the oviduct for sectioning. Note absence of inner cell mass (ICM) from the section of Tr vesicle. Scale bar: 50 µm.